Transgenic Generation

Whole zebrafish kdrl-gfp courtesy of Kevin Mouillesseaux

Whole zebrafish kdrl-gfp courtesy of Kevin Mouillesseaux

To generate a transgenic line:

  1. Prepare tools
    1. Identify the promoter for the gene of interest using standard molecular biology techniques
    2. Clone promoter upstream of a reporter

      The GATA:dsRed construct makes hemopoetic precursors (precursors to blood cells) glow red. Courtesy of Josh Blair, Bautch Lab

      (fluorescent protein or other reporter) using Tol2 kit

  2. Make mosaic fish using Tol2 transposase kit
  3. Evaluate expression pattern in F1 fish

That was fun! Let’s do it again! >> Model Benefits