The Zebrafish Aquaculture Core facility consists of a main aquaculture room and a three room suite in Taylor Hall containing an Injection room, Fluorescence Microscope room, and a Quarantine room.
Main Aquaculture Room (510 sqft ), containing:
- Centralized Aquatic System (Tecniplast)
- 18 adult aquaculture racks with a capacity of 3,080 liters and 15,400 fish
- two (2) nursery racks with a capacity of 198 liters and ~6,000 fry
- one (1) genotyping rack with a capacity of 288 individually housed fish
- drum filter
- three (3) water pumps
- two (2) heaters
- automated dosing systems for salt and pH adjustments
- carbon/particulate/UV filters
- 18 adult aquaculture racks with a capacity of 3,080 liters and 15,400 fish
- five (5) Tritone auto-feeding robots
- one (1) iSpawn for mass embryo generation
- a 6.5 cuft air incubator for embryo rearing
Injection Room (128 sqft), containing:
- three microinjection stations, each with a Leica S6E stereomicroscope and either a Drummond Nanoject II or Sutter Instruments Picospritzer III injector
- a Parker Instruments needle puller
- a cell transplantation apparatus
- a 13.2 cuft air incubator for embryo rearing
- three (3) 20 liter rotifer culture buckets
Fluorescence Microscopy Room (81 sqft ), equipped with:
- one Zeiss Axiozoom.V16 fluorescence stereomicroscope, equipped with color digital camera and computer
- one Leica M205C fluorescence stereomicroscope, equipped with high-speed monochrome fluorescence digital camera and computer
- a glassware disinfecting station
Quarantine Aquaculture Room (72 sqft), including:
- two (2) linked Aquarius racks from Aquatic Enterprises with a capacity of 480 liters and 2,400 fish
Nursery Room:
- one (1) Tecniplast aquaculture stand-alone rack with a capacity of 350 liters and 2,700 larval fish
- one (1) Tecniplast aquaculture stand-alone rack with a capacity of 425 liters and 875 juvenile fish
Screening Room: