The Zebrafish Aquaculture Core facility has two larger recirculating systems and several stand-alone systems, two procedure rooms, a fluorescence microscope room, and a feed prep room.

Main Aquaculture Room (cir. 2016; 510 sqft ), containing:

• Centralized Aquatic System (Tecniplast)
  • 18 adult aquaculture racks with a capacity of 3,080 liters and 15,400 fish
  • two (2) nursery racks with a capacity of 198 liters and ~6,000 fry
  • one (1) genotyping rack with a capacity of 288 individually housed fish
  • drum filter
  • three (3) water pumps
  • two (2) heaters
  • automated dosing systems for salt and pH adjustments
  • carbon/particulate/UV filters
• five (5) Tritone auto-feeding robots
• one (1) iSpawn for mass embryo generation

New Aquaculture System (cir. 2021), spanning (4) rooms:

• The system room contains double the Tecniplast equipment of the Main system
• The (3) holding rooms each have 7-8 racks
• The system has the capacity to double the number of racks and we have (2) extra rooms to build out when needed

Procedure Room I (128 sqft), containing:

• two (2) microinjection stations, each with a Leica S6E stereomicroscope and Sutter Instruments Picospritzer III injectors (Drummond Nanoject II also available)
• a Parker Instruments needle puller
• a cell transplantation apparatus
• a 13.2 cuft air incubator for embryo rearing

Fluorescence Microscopy Room (81 sqft ), equipped with:

• one (1) Zeiss Axiozoom.V16 fluorescence stereomicroscope, equipped with color digital camera and computer
• one (1) Leica M205C fluorescence stereomicroscope, equipped with high-speed monochrome fluorescence digital camera and computer
• one (1) Leica M16FA fluorescence stereomicroscope, equipped with color digital camera and computer

Killifish Aquaculture Room (72 sqft), including:

• Coming soon!
• One (1) Tecniplast aquaculture stand-alone rack with a capacity of 250 liters and 50 tanks.

Screening Room:

• four (4) Tecniplast aquaculture stand-alone racks with a capacity of 250 liters and 875 fish each
• one (1) Tecniplast aquaculture stand-alone rack with a capacity of 350 liters and 500 fish

Procedure Room II:

• One (1) survival surgery station
• Two (2) Leica S7E stereomicroscopes
• One (1) injector

Feed Prep Room:

• Maintain at least (2) continuous rotifer cultures

Quarantine Room, including:

• Two (2) Tecniplast aquaculture stand-alone racks with a capacity of 250 liters and 50 tanks each.
• Small incubator at 28C
• One (1) Leica S6E stereomicroscope